Ordinary light microscopy struggles to separate nearby objects about 200 to 300 nanometers apart.
- Shelf
- Science
- Kind
- Built from a recipe
- Your own
- Your own localizations
- Sound
- Yes
- Licenses
- CC BY 4.0
How to play, in the Playground
Pinch or scroll to zoom down to single molecules. Tap to see a thin slice at one depth.
Its controls
| Control | Kind | Range | Start |
|---|---|---|---|
| Show a slice at the depth you tap | Tap | ||
| A slice at one depth | Switch | Off | |
| Data | List | 7 choices | Microtubules and clathrin |
| Color by | List | 3 choices | Depth (z) |
| Depth scale | List | 2 choices | True (1×) |
| Precision | List | 3 choices | All localizations |
About it
Ordinary light microscopy struggles to separate nearby objects about 200 to 300 nanometers apart. Single-molecule localization microscopy finds the centers of isolated fluorescent blinks more precisely, then builds a picture from many positions. Localization precision is not the same as the final image's resolution, and several blinks may come from one molecule. Here each blink is drawn as a Gaussian whose width represents its reported uncertainty.
Choose microtubules and clathrin in a 12 µm cell region, a whole nucleus in 3D, or open a .smlm file or a CSV with positions and uncertainties in nanometers. Color by depth, time frame or channel. Depth scale shows true coordinates or stretches depth fourfold. Precision keeps all localizations or only those better than 5 or 3 nm. Pinch or scroll to zoom; tap for a 200 nm slice at that depth, then tap to restore all depths. Files stay on your device. This is a viewer, not a measurement tool.
Details
- Splats
- Opens at 200,000 at Mid Detail, 84,000 at Low, and up to 200,000 at Max
- Your own files
- .smlm, .csv, .txt, .zip
Sources and credits
| Work | By | License |
|---|---|---|
| Microtubules and clathrin in a Cos cell (ShareLoc.XYZ, 10.5281/zenodo.5507427) | Christophe Leterrier (Aix Marseille Université, CNRS, NeuroCyto), on ShareLoc.XYZ; a 12 µm square cut from the record (a subset) | CC BY 4.0 |
| Zola-3D NUP full nucleus (ShareLoc.XYZ, 10.5281/zenodo.7233696) | Andrey Aristov (Institut Pasteur), uploaded by Benoit Lelandais, on ShareLoc.XYZ; a random half of the localizations (a subset) | CC BY 4.0 |
| Xenopus laevis nuclear pore complex stained with WGA-ATTO520 (ShareLoc.XYZ, 10.5281/zenodo.7182237) | Anna Löschberger, on ShareLoc.XYZ; an 8 µm square cut from the record (a subset) | CC BY 4.0 |
| Actin with PhalloidinAF647 in COS7 (ShareLoc.XYZ, 10.5281/zenodo.5510661) | Sarah Aufmkolk, on ShareLoc.XYZ; a 10 µm square of one cell, 174,903 of its localizations (a subset) | CC BY 4.0 |
| Mitochondrial protein TOM22 in COS7 cells (ShareLoc.XYZ, 10.5281/zenodo.5512636) | Wei Ouyang, on ShareLoc.XYZ; a 15 µm square of one field, 219,846 of its localizations (a subset) | CC BY 4.0 |
| ZOLA-3D microtubules (ShareLoc.XYZ, 10.5281/zenodo.6861446) | Andrey Aristov, Benoit Lelandais and Christophe Zimmer (Institut Pasteur), on ShareLoc.XYZ; a 12 µm square, 159,785 of its localizations (a subset) | CC BY 4.0 |
